2325 pET30b-Ecoli-MutL
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Cloning of 2325 pET30b-Ecoli-MutL
[edit]Purpose: Cloning of2325 pET30b-Ecoli-MutL==
Date: 2015-1---
Notebook:0
Page:
Clone ID:2325
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from Escherichia coli str. K-12 substr. MG1655 genomic DNA
2325-Ecoli-MutL-Nde1-F tatcacatatgccaattcaggtcttaccgcca
2325-Ecoli MutL-Xho1-R TGGTGctcgagctcgagctcatctttcagggctt
Digest with Nde1 + Xho1
Colony Screening
[edit]Date: 2014_9
Make miniprep, use Nde1 + Xho1 to find positive clone
