2394 pT7A3E-RecO

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Cloning of 2394 pT7A3E-RecO

Purpose: Cloning of2394 pT7A3E-RecO==

Date: 2015_1

Notebook:0

Page:

Clone ID:2394

Experimenters: shanyi

Results

CLONING

PCR from Escherichia coli str. K-12 substr. MG1655 genomic DNA

2394-RecO-Nde1-F
tc gaa catatggaaggctggcagcgcgca
2394-RecO-Xho1-R
TGGTGctcgagttcataatgtgttttcaccgttc

digest with Nde1 + Xho1

Colony Screening

Date: 2015_1

Make miniprep, use Nde1 + Xho1 to find positive clone

Result intepretation/Summary

Conclusion

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Cloning