2394 pT7A3E-RecO
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Cloning of 2394 pT7A3E-RecO
[edit]Purpose: Cloning of2394 pT7A3E-RecO==
Date: 2015_1
Notebook:0
Page:
Clone ID:2394
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from Escherichia coli str. K-12 substr. MG1655 genomic DNA
2394-RecO-Nde1-F
tc gaa catatggaaggctggcagcgcgca
2394-RecO-Xho1-R
TGGTGctcgagttcataatgtgttttcaccgttc
digest with Nde1 + Xho1
Colony Screening
[edit]Date: 2015_1
Make miniprep, use Nde1 + Xho1 to find positive clone
