758 pEc2-PacI

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Cloning of758 pEc2-PacI

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Purpose: Cloning of758 pEc2-PacI==

Date: 2014-12_2015_1

Notebook:0

Page:

Clone ID:758

Experimenters:  shanyi

Results

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CLONING

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gene synthesis

clone to 2015 using Nde1 + Xho1


Colony Screening

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Date: 2015_1

Make miniprep, use Nde1 + Xho1 to find positive clone




Fix 758-2 and 758-3

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pcr

fragment 1 using 758-2 as template

758-PacI-1+758-PacI-4

fragment 1 using 758-3 as template

758-PacI-5+758-PacI-12


clone to 2015 using Nde1 + Xho1


Colony Screening

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Date: 2015_1

Make miniprep, use Nde1 + Xho1 to find positive clone



Result intepretation/Summary

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Conclusion

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