1004-pET8XHis-PKAalpha

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Cloning of 1004-pET8XHis-PKAalpha

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Purpose: Cloning of 1004-pET8XHis-PKAalpha by PCR

Date: 2012-2-7--

Notebook:003732

Page:

Clone ID:1004

Experimenters: yingying

Results

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cloning

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Date:2012-2-7

RTENOTITLE

 PCR : template is pSpeedET-PKAalapha

PKAalpha-Nco1-F + PKAalpha-BamH1-R

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band 3
 band 4

 PCR product
 286pET8XHis
digest enzymes
 Nco1+BamH1





Colony Screening

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Date: 2012-2-10

Make miniprep and digest with  Nco1+BamH1

RTENOTITLE

clone 1 to 6 may be right, need to sequence


Result intepretation/Summary

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  • <br>

Conclusion

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