1004-pET8XHis-PKAalpha
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Cloning of 1004-pET8XHis-PKAalpha
[edit]Purpose: Cloning of 1004-pET8XHis-PKAalpha by PCR
Date: 2012-2-7--
Notebook:003732
Page:
Clone ID:1004
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-2-7
PCR : template is pSpeedET-PKAalapha
PKAalpha-Nco1-F + PKAalpha-BamH1-R
| band 3 |
band 4 | |
| PCR product |
286pET8XHis | |
| digest enzymes |
Nco1+BamH1 | |
Colony Screening
[edit]Date: 2012-2-10
Make miniprep and digest with Nco1+BamH1
clone 1 to 6 may be right, need to sequence
Result intepretation/Summary
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Conclusion
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