Yidan 2016 10 04

Trec PCR Screening

1 ul of 10 uM primers for 25 ul PCR, 1 ul 10 mM dNTP
0.5ul Taq

PCR cycle:

1 min at 94 oC

35 cycles:
30 s at 94 oC
30 s at 55 oC
1 min at 72 oC

5 min at 72 oC