Yidan 2016 10 04
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Trec PCR Screening
1 ul of 10 uM primers for 25 ul PCR, 1 ul 10 mM dNTP
0.5ul Taq
PCR cycle:
1 min at 94 oC
35 cycles:
30 s at 94 oC
30 s at 55 oC
1 min at 72 oC
5 min at 72 oC
Trec PCR Screening
1 ul of 10 uM primers for 25 ul PCR, 1 ul 10 mM dNTP
0.5ul Taq
PCR cycle:
1 min at 94 oC
35 cycles:
30 s at 94 oC
30 s at 55 oC
1 min at 72 oC
5 min at 72 oC