Primery Amine Assay Copper
Primary Amine Assay using CuCl2
1 M CuCl2: 5 ul
PEI Solution: 10% (w/v) of PEI 1800, take 50 ul and diluted to 1000 ul,---> 0.5% (w/v), 0.5 g/100 ml
0.05g/10 ml, 0.005g/1ml, 5 mg/1ml, 5 ug/ul
| H2O, ul | PEI Std, ul | Amount | OD650 after 10 min | OD650 after 30 min |
| 95 | 5 | 25 ug | 0.071 |
0.173 |
| 90 | 10 | 50 ug | 0.161 |
0.25 |
| 80 | 20 | 100 ug | 0.386 |
0.47 |
| 70 | 30 | 150 ug | 0.508 |
0.585 |
| 60 | 40 | 200 ug | 0.62 |
0.725 |
| 50 | 50 | 250 ug | 0.742 |
0.831 |
| 40 | 60 | 300 ug | 0.881 |
0.966 |
| 30 | 70 | 350 ug | 0.995 |
1.059 |
| 20 | 80 | 400 ug | 1.143 |
1.212 |
| 10 | 90 | 450 ug | 1.265 |
1.339 |
| 5 | 95 | 475 ug | 1.357 |
1.406 |
PHP-DSP-Pyrozale: 5 ul 0.409; 10 ul: 0.653
