Primery Amine Assay Copper

Primary Amine Assay using CuCl_²

1 M CuCl2: 5 ul

PEI Solution: 10% (w/v) of PEI 1800, take 50 ul and diluted to 1000 ul,---> 0.5% (w/v), 0.5 g/100 ml

0.05g/10 ml, 0.005g/1ml, 5 mg/1ml, 5 ug/ul 

H2O, ul	PEI Std, ul	Amount	OD650 after 10 min	OD650 after 30 min
95	5	25 ug	0.071	0.173
90	10	50 ug	0.161	0.25
80	20	100 ug	0.386	0.47
70	30	150 ug	0.508	0.585
60	40	200 ug	0.62	0.725
50	50	250 ug	0.742	0.831
40	60	300 ug	0.881	0.966
30	70	350 ug	0.995	1.059
20	80	400 ug	1.143	1.212
10	90	450 ug	1.265	1.339
5	95	475 ug	1.357	1.406

PHP-DSP-Pyrozale: 5 ul 0.409; 10 ul: 0.653