Glycine HCl Buffer
0.1 M GLYCINE–HCL; PH 2.2–3.6, PKA = 2.35
Glycine MW: 75.07
0.2 M Weigh 7.5 g glycine , add DI water to 500 ml
0.2 M HCl: 8.6 ml of Concentrated HCl (N=11.65), add DI water to 500 ml.
0.1 M Glycine-HCl: Add x ml 0.2M HCl to 500 ml 0.2 M Glycine, then add DI water to 1000 ml
| x (ml) |
pH |
| 50 |
3.6 |
| 64 | 3.4 |
| 82 | 3.2 |
| 168 | 2.8 |
| 242 | 2.6 |
To make 1000 ml 0.2 M Glycine-HCl, pH 2.6: 15 g Glycine, + 5 ml conc HCl, DI water to 1 L.