Glycine HCl Buffer

0.1 M GLYCINE–HCL; PH 2.2–3.6, PKA = 2.35

Glycine MW: 75.07

0.2 M Weigh 7.5 g glycine , add DI water to 500 ml

0.2 M HCl: 8.6 ml of Concentrated HCl (N=11.65), add DI water to 500 ml.

0.1 M Glycine-HCl: Add x ml 0.2M HCl to 500 ml 0.2 M Glycine, then add DI water to 1000 ml

x (ml)	pH
50	3.6
64	3.4
82	3.2
168	2.8
242	2.6

To make 1000 ml 0.2 M Glycine-HCl, pH 2.6: 15 g Glycine, + 5 ml conc HCl, DI water to 1 L.