Daniel 2017 06 19

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1. digest, ligate,transformation vector 818 and MBP

lane 1,2 are vector 818, lane 3,4 are MBP

2. PCR 823

	1	2	3	4	5	6
enzyme	-	-	-	+	+	+
template	-	-	+	-	+	+

3. PCR colony screening 827.826

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