Daniel 2017 06 19
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1. digest, ligate,transformation vector 818 and MBP
lane 1,2 are vector 818, lane 3,4 are MBP
2. PCR 823
| 1 | 2 | 3 | 4 | 5 | 6 | |
| enzyme | - | - | - | + | + | + |
| template | - | - | + | - | + | + |
3. PCR colony screening 827.826