353-pET28TaqMuts

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Cloning of TaqMuts to pET28b

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Purpose: cloning of TaqMuts to pET28b (353).

Date: 2011-9-4 to 2011-9- 

Notebook:

Page:

Clone ID:

Experimenters: Paul

Results

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PCR

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Use

Primer-F Taq-Muts-F cata cat atggaaggcat gctgaagggc
Primer-R Taq-Muts-R cgatgaattctcaccccttcatggtatccaag

2.5 ul DMSO in 50 ul reaction.

Phusion HiFi Polymerase

2.5 min extension, 25 cycles

--Paul.xu 19:06, 4 September 2011 (EST)

Colony Screening

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Result intepretation/Summary

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Conclusion

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Restriction digestion

Shows 

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