353-pET28TaqMuts
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Cloning of TaqMuts to pET28b
[edit]Purpose: cloning of TaqMuts to pET28b (353).
Date: 2011-9-4 to 2011-9-
Notebook:
Page:
Clone ID:
Experimenters: Paul
Results
[edit]PCR
[edit]Use
Primer-F Taq-Muts-F cata cat atggaaggcat gctgaagggc
Primer-R Taq-Muts-R cgatgaattctcaccccttcatggtatccaag
2.5 ul DMSO in 50 ul reaction.
Phusion HiFi Polymerase
2.5 min extension, 25 cycles
--Paul.xu 19:06, 4 September 2011 (EST)
Colony Screening
[edit]Result intepretation/Summary
[edit]Conclusion
[edit]Restriction digestion
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