MS 6 27 2017

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Culture 1995 Kfu 200ml.
Culture 1458 KOD 8L, induced with IPTG.

Homogenize 1518 T3 ligase, load onto Ni column.

Elute 1697 GST-wt3C with Buffer B 06/27/2017

-0.21
 -0.21
 -0.21
 0.00
 4.41
 (1~5)
3.66
 1.16
 0.60
 0.35
 0.24
 (6~10)
0.19
 0.15
 0.13
 0.06
 0.03
 (11~15)

collect 5~11.

Load 1697 GST-wt3C onto QHP column.


Elute 1942 phi29

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