MS 6 27 2017
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Culture 1995 Kfu 200ml.
Culture 1458 KOD 8L, induced with IPTG.
Homogenize 1518 T3 ligase, load onto Ni column.
Elute 1697 GST-wt3C with Buffer B 06/27/2017
| -0.21 |
-0.21 |
-0.21 |
0.00 |
4.41 |
(1~5) |
| 3.66 |
1.16 |
0.60 |
0.35 |
0.24 |
(6~10) |
| 0.19 |
0.15 |
0.13 |
0.06 |
0.03 |
(11~15) |
collect 5~11.
Load 1697 GST-wt3C onto QHP column.
Elute 1942 phi29
