837 pMiniT-Hia5

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6/23/2017

Colony PCR screening

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6/24/17

Miniprep, digestion with EcoRV

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miniprep purify by isopropanol precipitation


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Miniprep purified by spin column

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sequencing:

clone #2 has deletion mutation

clone #4 high background


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837 clone #4, tranform DH5alpha with plasmid, make miniprep, digest with HindIII + Xba1

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