Important Enzymes
| Name |
ID |
PI/MW |
Antibiotics | Thermostable | Expression Screening | Status | Lot # | Yield(mL) |
Final Concentration | Comment | QC Page | |
| VPol |
1811 |
6.63 / 69.2 |
K | Y, moderate |
complete ? |
105-1811 |
need to run SDS-PAGE |
http://excellgen.net/eln/index.php/MS_2_22_2017 | ||||
| VPol |
1811 | 6.63 / 69.2 |
K | Y, moderate |
complete |
138-1811 |
10.0 | 36.0mg/ml |
http://excellgen.net/eln/index.php/MS_7_6_2017 | |||
| Kfu |
1995 |
7.87 / 99.3 |
K | Yes |
complete ? |
107-1995 |
5.3 |
3.27mg/ml |
need to run SDS-PAGE |
http://excellgen.net/eln/index.php/MS_3_1_2017 | ||
| Kfu |
1995 | 7.87 / 99.3 |
K | Yes | complete |
137-1995 |
4.5 | 4.18mg/ml |
http://excellgen.net/eln/index.php/MS_7_5_2017 | |||
| yPolyA-Pol |
2295 | 7.63 / 66.2 |
K | N |
complete ? |
108-2295 |
6/14.3 |
0.69/0.83 |
need ti run SDS-PAGE most likely no purification, need to do expression screening |
http://excellgen.net/eln/index.php/MS_3_20_2017 | ||
| yPolyA-Pol |
2295 |
7.63 / 66.2 |
K | N | http://excellgen.net/eln/index.php/MS_5_25_2017 |
complete |
131-2295 |
2.0 | 4.7mg/ml |
http://excellgen.net/eln/index.php/MS_6_22_2017 | ||
| iFLP |
2409 |
9.89 / 97.1 |
K | N |
complete ? |
109-2409 |
10.8 |
0.63 mg/ml |
need to run SDS-PAGE |
http://excellgen.net/eln/index.php/MS_3_14_2017 | ||
| KOD |
1458 |
7.91 / 92.2 |
K | Y |
complete ? |
110-1458 |
5.5/6 |
7.44/3.24 |
need to run SDS-PAGE |
http://excellgen.net/eln/index.php/MS_4_5_2017 | ||
| KOD |
1458 |
7.91 / 92.2 |
K |
Y | complete |
136-1458 |
4.5 | 14.27mg/ml |
http://excellgen.net/eln/index.php/MS_7_4_2017 | |||
| T5-Exo |
1567 |
5.55 / 34.5 |
K | N |
complete no purification |
111-1567 |
4.8 |
0.2mg/ml |
need to expression screening |
http://excellgen.net/eln/index.php/MS_4_18_2017 | ||
| T5-Exo |
1567 |
5.55 / 34.5 |
K | N | complete |
140-1567 |
8.0 | 1.5mg/ml |
http://excellgen.net/eln/index.php/MS_7_11_2017 | |||
| T4 DNA Ligase |
1483 |
6.63 / 57.3 |
K | N |
complete ? |
112-1483 |
23.0 |
8.10mg/ml |
not pure, need further purification |
http://excellgen.net/eln/index.php/MS_4_26_2017 | ||
| T4 DNA Ligase |
1483 |
6.63 / 57.3 |
K | N | complete |
130-1483 |
2.0 | 32mg/ml |
http://excellgen.net/eln/index.php/MS_6_21_2017 | |||
| MthRnl |
1315 |
5.81 / 45.3 |
k | N |
complete, not yet |
113-1315 |
10.3 | 2.30mg/ml |
not pure NEB said it does not work |
http://excellgen.net/eln/index.php/MS_4_20_2017 | ||
| Mth RNA Ligase |
1315 |
5.81 / 45.3 |
K | Y | complete |
122-1315 |
18.8 |
6.50 mg/ml |
http://excellgen.net/eln/index.php/MS_5_19_2017 | |||
| T4DNAPol |
1765 |
6.19 / 105.9 |
K | N | complete? |
114-1765 |
17.5 |
2.42mg/ml |
don't not know if protein is correct |
http://excellgen.net/eln/index.php/MS_4_26_2017 | ||
| TthPol (for RT-qPCR) |
483 |
6.21 / 96.1 | K | Yes | failed | 115-483 |
not pure | http://excellgen.net/eln/index.php/MS_5_1_2017 | ||||
| TthPol (for RT-qPCR) |
483 |
6.21 / 96.1 |
K | Yes | http://excellgen.net/eln/index.php/MS_5_26_2017 |
complete |
125-483 |
13.0 | 1.11mg/ml |
http://excellgen.net/eln/index.php/MS_6_6_2017 | ||
| T7 DNA Ligase |
1464 |
5.27 /41.0 |
K | N | failed | 116-1464 |
not pure | http://excellgen.net/eln/index.php/MS_5_3_2017 | ||||
| T7 DNA Ligase |
1464 |
5.27 /41.0 |
K | N | http://excellgen.net/eln/index.php/MS_5_26_2017 |
complete |
126-1464 |
4.5 | 23.2mg/ml |
http://excellgen.net/eln/index.php/MS_6_9_2017 | ||
| TdT bovine |
1946 |
7.67 / 45.7 |
K | N | failed | 117-1946 |
not pure | http://excellgen.net/eln/index.php/MS_5_9_2017 | ||||
| TdT bovine |
1946 |
7.67 / 45.7 |
K | N | http://excellgen.net/eln/index.php/MS_5_26_2017 |
complete |
127-1946 |
2.0 | 3.3mg/ml |
http://excellgen.net/eln/index.php/MS_6_14_2017 | ||
| ToughTaq |
1497 | 7.40 / 72.2 |
K | Y | failed | 118-1497 |
not pure | http://excellgen.net/eln/index.php/MS_5_9_2017 | ||||
| ToughTaq |
1497 |
7.40 / 72.2 |
K | Y | http://excellgen.net/eln/index.php/MS_5_26_2017 |
complete |
128-1497 |
2.7 | 4.5mg/ml |
http://excellgen.net/eln/index.php/MS_6_15_2017 | ||
| T3 Ligase |
1518 |
5.62 / 41.6 |
K | N | complete |
119-1518 |
6.5 |
2.35mg/ml |
not pure |
http://excellgen.net/eln/index.php/MS_5_15_2017 | ||
| T3 Ligase |
1518 |
5.62 / 41.6 |
K | N | http://excellgen.net/eln/index.php/MS_6_14_2017 |
complete |
132-1518 |
12.0 | 0.36mg/ml |
not pure | http://excellgen.net/eln/index.php/MS_6_22_2017 | |
| T3 Ligase |
1518 |
5.62 / 41.6 |
K | N | complete |
135-1518 |
3.5 | 2.50mg/ml |
http://excellgen.net/eln/index.php/MS_6_29_2017 | |||
| sso7d Native ToughTaq |
1542 |
7.36 / 72.0 |
K | Y | complete |
120-1542 |
7.7 | 3.84mg/ml |
http://excellgen.net/eln/index.php/MS_5_15_2017 | |||
| Pfu-sso7d |
1715 |
8.47 / 98.6 |
K | Y | complete |
121-1715 |
7.0 | 4.10 mg/ml |
http://excellgen.net/eln/index.php/MS_5_19_2017 | |||
| pT7A3-Tth-Ligase | 1782 |
6.56 / 79.1 |
K | 15 min 75 oC |
http://excellgen.net/eln/index.php/MS_5_25_2017 |
complete |
124-1782 |
10.0 | 10.64mg/ml |
not pure | http://excellgen.net/eln/index.php/MS_6_6_2017 | |
| pT7A3-TaqDNALigase |
1498 | 6.56 / 79.1 |
K |
15 min 75 oC |
http://excellgen.net/eln/index.php/MS_5_25_2017 |
complete | 129-1498 | 3.3 | 1.5mg/ml |
http://excellgen.net/eln/index.php/MS_6_15_2017 | ||
| pT7A3-new-phi29 |
1942 |
5.72 / 70.6 |
K | N | http://excellgen.net/eln/index.php/MS_6_14_2017 |
complete |
133-1942 | 3.0 | 1.07mg/ml |
http://excellgen.net/eln/index.php/MS_6_28_2017 | ||
| pCold-GST-(3c)-wt3C |
1697 | 8.48 / 21.4 |
K | N | induce at 17~20 oC |
complete | 134-1697 |
4.0 | 3.03mg/ml |
http://excellgen.net/eln/index.php/MS_6_28_2017 | ||
| Tn5 transposase |
2462 | 8.41 / 83.8 |
K | N | complete |
139-2462 |
5.5 | 1.48mg/ml | http://excellgen.net/eln/index.php/MS_7_7_2017 | |||
| pET30b-DeepVent-sso7d |
423 |
8.69 / 99.5 |
K | Y, 30 min 75 oC |
complete |
141-423 |
8.0 | 1.50mg/ml |
http://excellgen.net/eln/index.php/MS_7_12_2017 | |||
| T4DNAPol |
1765 |
6.19 / 105.9 |
k |
N |
http://excellgen.net/eln/index.php/MS_6_14_2017 |
|||||||
| TdT human | 1509 |
8.55 / 59.9 |
k | N | ||||||||
| ToughTaq |
1722 |
7.36 / 72 | K |
Y | ||||||||
| pT7A3-6His-GST-i3C |
1601 |
|
K | N | http://excellgen.net/eln/index.php/MS_6_14_2017 |
|||||||
| pT7A3-phi29wt-sso7d |
1926 |
8.70 / 75.7 |
K | N | ||||||||
| pT7A3E-PBCV-1-Lig |
2196 |
6.90 / 35.6 |
K | N | http://excellgen.net/eln/index.php/MS_5_25_2017 |
|||||||
|
1613 | 6.99 / 47.4 |
induce at 17~20 oC |
|||||||||
|
pET30-HA-Tn5-6His or pET28b-Tn5 |
2474 or 2443 |
9.54 / 57.0 |
K | |||||||||
| pET28b-T7-Endo1 |
1225 |
|||||||||||