Important Enzymes

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Name
ID
PI/MW
Antibiotics Thermostable Expression Screening Status Lot # Yield(mL)
Final Concentration Comment QC Page
VPol
1811
6.63 / 69.2
K Y, moderate

complete

?

105-1811


need to run SDS-PAGE
http://excellgen.net/eln/index.php/MS_2_22_2017
VPol
1811 6.63 / 69.2
K Y, moderate

complete
138-1811
10.0 36.0mg/ml

http://excellgen.net/eln/index.php/MS_7_6_2017
Kfu
1995
7.87 / 99.3
K Yes

complete

?

107-1995
5.3
3.27mg/ml
need to run SDS-PAGE
http://excellgen.net/eln/index.php/MS_3_1_2017
Kfu
1995 7.87 / 99.3
K Yes
complete
137-1995
4.5 4.18mg/ml

http://excellgen.net/eln/index.php/MS_7_5_2017
yPolyA-Pol
2295 7.63 / 66.2
K N

complete

?

108-2295
6/14.3
0.69/0.83

need ti run SDS-PAGE

most likely no purification, need to do expression screening

http://excellgen.net/eln/index.php/MS_3_20_2017
yPolyA-Pol
2295
7.63 / 66.2
K N http://excellgen.net/eln/index.php/MS_5_25_2017
complete
131-2295
2.0 4.7mg/ml

http://excellgen.net/eln/index.php/MS_6_22_2017
iFLP
2409
9.89 / 97.1
K N

complete

?

109-2409
10.8
0.63 mg/ml
need to run SDS-PAGE
http://excellgen.net/eln/index.php/MS_3_14_2017
KOD
1458
7.91 / 92.2
K Y

complete

?

110-1458
5.5/6
7.44/3.24
need to run SDS-PAGE
http://excellgen.net/eln/index.php/MS_4_5_2017
KOD
1458
7.91 / 92.2
K
Y
complete
136-1458
4.5 14.27mg/ml

http://excellgen.net/eln/index.php/MS_7_4_2017
T5-Exo
1567
5.55 / 34.5
K N

complete

no purification

111-1567
4.8
0.2mg/ml
need to expression screening
http://excellgen.net/eln/index.php/MS_4_18_2017
T5-Exo
1567
5.55 / 34.5
K N
complete
140-1567
8.0 1.5mg/ml

http://excellgen.net/eln/index.php/MS_7_11_2017
T4 DNA Ligase
1483
6.63 / 57.3
K N

complete

?

112-1483
23.0
8.10mg/ml
not pure, need further purification
http://excellgen.net/eln/index.php/MS_4_26_2017
T4 DNA Ligase
1483
6.63 / 57.3
K N
complete
130-1483
2.0 32mg/ml

http://excellgen.net/eln/index.php/MS_6_21_2017
MthRnl
1315
5.81 / 45.3
k N

complete,

not yet

113-1315
10.3 2.30mg/ml

not pure

NEB said it does not work

http://excellgen.net/eln/index.php/MS_4_20_2017
Mth RNA Ligase
1315
5.81 / 45.3
K Y
complete
122-1315
18.8
6.50 mg/ml

http://excellgen.net/eln/index.php/MS_5_19_2017
T4DNAPol
1765
6.19 / 105.9
K N
complete?
114-1765
17.5
2.42mg/ml
don't not know if protein is correct
http://excellgen.net/eln/index.php/MS_4_26_2017
TthPol (for RT-qPCR)
483
6.21 / 96.1 K Yes
failed 115-483


not pure http://excellgen.net/eln/index.php/MS_5_1_2017
TthPol (for RT-qPCR)
483
6.21 / 96.1
K Yes http://excellgen.net/eln/index.php/MS_5_26_2017
complete
125-483
13.0 1.11mg/ml

http://excellgen.net/eln/index.php/MS_6_6_2017
T7 DNA Ligase
1464
5.27 /41.0
K N
failed 116-1464


not pure http://excellgen.net/eln/index.php/MS_5_3_2017
T7 DNA Ligase
1464
5.27 /41.0
K N http://excellgen.net/eln/index.php/MS_5_26_2017
complete
126-1464
4.5 23.2mg/ml

http://excellgen.net/eln/index.php/MS_6_9_2017
TdT bovine
1946
7.67 / 45.7
K N
failed 117-1946


not pure http://excellgen.net/eln/index.php/MS_5_9_2017
TdT bovine
1946
7.67 / 45.7
K N http://excellgen.net/eln/index.php/MS_5_26_2017
complete
127-1946
2.0 3.3mg/ml

http://excellgen.net/eln/index.php/MS_6_14_2017
ToughTaq
1497 7.40 / 72.2
K Y
failed 118-1497


not pure http://excellgen.net/eln/index.php/MS_5_9_2017
ToughTaq
1497
7.40 / 72.2
K Y http://excellgen.net/eln/index.php/MS_5_26_2017
complete
128-1497
2.7 4.5mg/ml

http://excellgen.net/eln/index.php/MS_6_15_2017
T3 Ligase
1518
5.62 / 41.6
K N
complete
119-1518
6.5
2.35mg/ml
not pure
http://excellgen.net/eln/index.php/MS_5_15_2017
T3 Ligase
1518
5.62 / 41.6
K N http://excellgen.net/eln/index.php/MS_6_14_2017
complete
132-1518
12.0 0.36mg/ml
not pure http://excellgen.net/eln/index.php/MS_6_22_2017
T3 Ligase
1518
5.62 / 41.6
K N
complete
135-1518
3.5 2.50mg/ml

http://excellgen.net/eln/index.php/MS_6_29_2017
sso7d Native ToughTaq
1542
7.36 / 72.0
K Y
complete
120-1542
7.7 3.84mg/ml

http://excellgen.net/eln/index.php/MS_5_15_2017
Pfu-sso7d 
1715
8.47 / 98.6
K Y
complete
121-1715
7.0 4.10 mg/ml

http://excellgen.net/eln/index.php/MS_5_19_2017
 pT7A3-Tth-Ligase 1782
6.56 / 79.1
K 15 min 75 oC
http://excellgen.net/eln/index.php/MS_5_25_2017
complete
124-1782
10.0 10.64mg/ml
not pure http://excellgen.net/eln/index.php/MS_6_6_2017
pT7A3-TaqDNALigase
1498 6.56 / 79.1
K
15 min 75 oC
http://excellgen.net/eln/index.php/MS_5_25_2017
complete 129-1498 3.3 1.5mg/ml

http://excellgen.net/eln/index.php/MS_6_15_2017
pT7A3-new-phi29
1942
5.72 / 70.6
K N http://excellgen.net/eln/index.php/MS_6_14_2017
complete
133-1942 3.0 1.07mg/ml

http://excellgen.net/eln/index.php/MS_6_28_2017
pCold-GST-(3c)-wt3C
1697 8.48 / 21.4
K N induce at 17~20 oC
complete 134-1697
4.0 3.03mg/ml

http://excellgen.net/eln/index.php/MS_6_28_2017
Tn5 transposase
2462 8.41 / 83.8
K N
complete
139-2462
5.5 1.48mg/ml
http://excellgen.net/eln/index.php/MS_7_7_2017
pET30b-DeepVent-sso7d
423
8.69 / 99.5
K Y, 30 min 75 oC

complete
141-423
8.0 1.50mg/ml

http://excellgen.net/eln/index.php/MS_7_12_2017
T4DNAPol
1765
6.19 / 105.9
k
N
http://excellgen.net/eln/index.php/MS_6_14_2017






TdT human 1509

8.55 / 59.9

k N






ToughTaq
1722
7.36 / 72 K
Y






 pT7A3-6His-GST-i3C
1601 
6.41 / 50.8
K N http://excellgen.net/eln/index.php/MS_6_14_2017






pT7A3-phi29wt-sso7d
1926
8.70 / 75.7
K N






pT7A3E-PBCV-1-Lig
2196
6.90 / 35.6
K N http://excellgen.net/eln/index.php/MS_5_25_2017






pET28-cold-GST-3C
1613 6.99 / 47.4


induce at 17~20 oC

http://excellgen.net/eln/index.php/MS_5_25_2017







 pET30-HA-Tn5-6His

or  pET28b-Tn5

2474 or 2443
9.54 / 57.0
K







pET28b-T7-Endo1
1225