2046-pET28b-Tat-NLS-yFlp-6H

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Cloning of 2046-pET28b-Tat-NLS-yFlp-6H

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Purpose: Cloning of 2046-pET28b-Tat-NLS-yFlp-6H

Date: 2014-1-30---2014-2-3

Notebook:0

Page:

Clone ID:2046

Experimenters: yingying

Results

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cloning

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RTENOTITLE

fragment 1:

template:pET28b-yFLP
yFlp-Nde1-F
yFlp-R1


fragment 2:

template:pET28b-yFLP
yFlp-F1
yFlp-6His-Xho1-R

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pcr1+pcr2

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lane
 5
 6

 pcr
 pET28b

 Nde1 + Xho1


Colony Screening

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Date: 2014-2-3

Make miniprep and digest with Nde1 + Xho1

RTENOTITLE


--Paul.xu (talk) 11:26, 20 November 2017 (EST)

Result intepretation/Summary

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Conclusion

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