2046-pET28b-Tat-NLS-yFlp-6H

Cloning of 2046-pET28b-Tat-NLS-yFlp-6H

Purpose: Cloning of 2046-pET28b-Tat-NLS-yFlp-6H

Date: 2014-1-30---2014-2-3

Notebook:0

Page:

Clone ID:2046

Experimenters: yingying

Results

cloning

fragment 1:

template:pET28b-yFLP
yFlp-Nde1-F
yFlp-R1

fragment 2:

template:pET28b-yFLP
yFlp-F1
yFlp-6His-Xho1-R

pcr1+pcr2

lane	5	6
	pcr	pET28b
	Nde1 + Xho1

Colony Screening

Date: 2014-2-3

Make miniprep and digest with Nde1 + Xho1

--Paul.xu (talk) 11:26, 20 November 2017 (EST)