3082 Cel1

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subcloning from pJet1.2 to pET28b

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pJet1.2 vector, 2046 is used as pET28b vector

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screening plasmid  pET28b-Cel1, 2082 is actually 3082, look like there is no insert

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3082 in pET28b: Nde1+ Xho1, only 5 colonies, toxic, no good

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