3082 Cel1

subcloning from pJet1.2 to pET28b

pJet1.2 vector, 2046 is used as pET28b vector

screening plasmid  pET28b-Cel1, 2082 is actually 3082, look like there is no insert

3082 in pET28b: Nde1+ Xho1, only 5 colonies, toxic, no good