Incentive pay schemes

#	Task	Pay ($)	Deliverables	Note
1	Come to work	10	Work ~8 hours in the lab, filll tips, make gels, medium etc
2	Shipping	5	Enter PO order into database, print labels, prepare package, deliver to mail room or FedEx office
3	Colony PCR Screening	5	see protocol	for Paul's clonining task
4	Miniprep & Restriction digestion	10	see protocol	for Paul's clonining task
5	PCR cloning into pMiniT	20	including PCR screening, miniprep, digestion, sequencing, glycerol stock, documentation etc clone must be confrimed by sequencing
6	PCR cloning directly into expression vector	35	including PCR screening, miniprep, digestion, sequencing, glycerol stock, documentation etc clone must be confrimed by sequencing
7	Subcloning	15	including PCR screening, miniprep, digestion, sequencing, glycerol stock, documentation etc clone must be confrimed by  PCR, digestion  or sequencing
8	Cloning gene fragment into pMiniT	20	including PCR screening, miniprep, digestion, sequencing, glycerol stock, documentation etc clone must be confrimed by sequencing
9	Gene synthesis and clone into pMiniT	30	including PCR screening, miniprep, digestion, sequencing, glycerol stock, documentation etc clone must be confrimed by sequencing
10	Protein Expression screening	10	must be  >5 clones per experiment
11	Protein purification	150	from at least 8 L culture, purified protein by Ni column and then Q or SP or other columns, concentrated, dialysed. Purity should be >90% by SDS-PAGE.
12	3rd column chromatography	30	further purification using one additional column
13	Cell culture	5	change medium, take care of cultured cells
14	Transfection	10
15	Real Time PCR	5