3168 pCEH0-His-VH-mIgG2a

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Subclone of 3168

Retsriction enzymes:BsrGI+NheI

Time:30MIn

Buffer:Fastdigest

Results:good, combine and do the step.

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Miniprep of 3168_3132_3161

3168:1-4 BsrGI+NheI

3132:5-8 BsrGI+NheI

3161:9-12 NdeI+XhoI

Buffer:Fastdigest

Time:30Min

Result:#7 seems good, so sent to do sequencing, the rest of them are suspicious

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Date:3/14/2018

Miniprep digestion_3161_3168

3161:1,2 NdeI+XhoI Buffer:Cutsmart

3168:3 BsrGI+NheI

Buffer:NEB 2

Time:40min

Result:seems good, do sequencing

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