3168 pCEH0-His-VH-mIgG2a
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Subclone of 3168
Retsriction enzymes:BsrGI+NheI
Time:30MIn
Buffer:Fastdigest
Results:good, combine and do the step.
Miniprep of 3168_3132_3161
3168:1-4 BsrGI+NheI
3132:5-8 BsrGI+NheI
3161:9-12 NdeI+XhoI
Buffer:Fastdigest
Time:30Min
Result:#7 seems good, so sent to do sequencing, the rest of them are suspicious
Date:3/14/2018
Miniprep digestion_3161_3168
3161:1,2 NdeI+XhoI Buffer:Cutsmart
3168:3 BsrGI+NheI
Buffer:NEB 2
Time:40min
Result:seems good, do sequencing
