Completed Clones
Need to sequence
[edit]=====finished but have not sequenced yet======
| # | Name | Status | Date | picture | Vector# | Enzymes | Size | Antibiotics | Method | Note | Next Action | QC_sequence clone# |
| 2509 | pT7A30-pMBP-Cid |
result | Nde1 -Xho1 |
978 | K | Subclone | compared with + | re-doing | ||||
| 3204 | done | |||||||||||
| 3210 | pT7A30-Sumo-Ov-ASP1-BCMA |
result | 1801 | Nde1-EcoR1 |
999 | K | Subclone | compared with + | sequencing T7 Term;done |
|||
| 3215 | pCMV5-MICA-a-Avi-6H-BirA |
result | 3237 | Amp | subclone | compared with +and 3237 Vector digestion |
done | |||||
| 3199 | pT7A3-GST-Avitag |
result | 3237 | Amp | subclone | compared with +and 3237 Vector digestion |
done | |||||
| 3196 | pCMV5-CD47-Avi-6H-BirA |
result | 3237 | Amp | subclone | compared with +and 3237 Vector digestion |
done | |||||
| 941 | pT7A3-pMBP-T7-Endo-1 |
result | 947 | K | subclone | comnpare with + | done | |||||
| 129 | ||||||||||||
| 994 | ||||||||||||
| 3197 | ||||||||||||
| 995 |
Completed and sequenced
[edit]==========completed and sequenced==============
| ID | Status | Date | Name | Progress |
Date | Supervisor's Note | Clone # | Status |
| 3127 |
pET28b-qBeta-qBeta-6XHis |
PCR qbeta and clone back into 3097 | already finished on 5/3/2018,sequenced | glycerol stock ? expression screening? | GS made | |||
| 938 | PNGase F, | pJet1.2, sequencing is good, need to subclone into pET30b | finished | glycerol stock ? expression screening? |
GS made | |||
| 939 pET30b-Tte-ExoIII | Tte-Exo III |
pJet1.2 sequencing is good, need to subclone into pET30b |
finished | glycerol stock ? expression screening? |
GS made | |||
| 942 pET28b-pMBP-VV | VVN endonuclease |
already in pJet1.2 need to check by digestion
|
finished | glycerol stock ? expression screening? |
GS made | |||
| 3122 | Crm197, secreted | already in pJet1.2,subclone to pET30b(vector#500), alrady finished | done,finished | glycerol stock ? expression screening? |
GS made | |||
| 945 |
pT7A30-pMBP |
PCR from 1281 pMAL-p5E or 1282 pMAL-p5E-AvPAL sequencing is good, completed |
sequencing verfied |
GS made | ||||
| 947 |
pT7A30-6His-pMBP |
PCR from 1281 pMAL-p5E or 1282 pMAL-p5E-AvPAL (as template) sequencing is good, completed |
sequencing verfied |
GS made | ||||
| 944 | pET30b-pfu-exo- |
944 pET30b-pfu-exo digestion , sequencing result: finished | glycerol stock ? expression screening? |
GS made | ||||
| 824 |
0 | pET28b-VVTopo |
subclone from 832 pMiniT-VVTopo using Nde1 + Xho1 in pET28 vector, sequenced |
glycerol stock ? expression screening? |
GS made | |||
| 993 |
pCold-GST-M13-Gene8 |
+993pCold-GST-M13-Gene8digestion | sequencing with T7 Term, expression screening? |
|||||
| 992 |
pCold-GST-V5 |
+992pCold-GST-V5digestion | sequencing with T7 Term, then expression screening | |||||
| 991 |
pCold-GST-Flag |
+991pCold-GST-Flagdigestion | sequencing with T7 Term, then expression screening |
|||||
| 990 |
pCold-GST-HA |
+990pCold-GST-HAdigestion | sequencing with T7 Term, then expression screening |
|||||
| 989 |
pCold-GST-Myc |
+989pCold-GST-Mycdigestion | sequencing with T7 Term, then expression screening |
|||||
| 985 |
pCold-GST-3C-AP205-M13-g3p |
985pCold-GST-3C-AP205-M13-g3pdigestion, good sequenced, | expression screening |
|||||
| 997 | pT7A3-Qbeta |
997pT7A3-QbetaDigestion , sequenced , good, completed | no his tag, do not need to do expression screening | |||||
| 986 | pT7A3-6His-NLS-Cas9-NLS-6xHis |
986pT7A3-6His-NLS-Cas9-NLS-6xHis digestion looks good, good, sequenced | expression screening? |
GS made | ||||
984 |
pT7A3-6H-SBP-SUMO-3C | 6/6/18 sequencing verified 6/5/2018 sequenced |
expression screening? |
GS made | ||||
| 3192 | pCMV5-PD-1-Avi-6H-BirA |
sequencing good, completed | make miniprep from 15 ml |
GS made | ||||
| 3166 |
pCMV-mIgG2b-HC |
sequencing good, completed |
make miniprep from 15 ml |
GS made | ||||
| # | Name | Status | Date | picture | Vector# | Enzymes | Size | Antibiotics | Method | Note | Next Action | QC_sequence clone# | Final Status |
| 3233 | pCMV5-IL2-CD47-(3c)-Fc-WPRE-OriP |
result | 1393 | Nhe1-KpnI |
375 | Amp | Subclone | compared with + | sequencing good, completed |
#1 |
GS made | ||
| 2503 | pCold-GST-VVTopo |
result | 3161 | Nde1 -Xho1 |
951 | K | Subclone | compared with + | sequencing good, completed |
#4 |
GS made | ||
| 3194 | pCMV5-IL2-BCMA-(3c)-Fc-WPRE-OriP |
result | 1393 | Nhe1-KpnI |
174bp | Amp | PCR | sequencing good, completed |
#2 |
GS made | |||
| 3215 | pCMV5-MICA-a-Avi-6H-BirA |
result | 3192 | Nhe1-Kpn1 | 285bp | Amp | PCR | sequencing insert is good, subclone to 3232 |
#1 | sub | |||
| 3204 | pCMV5-OX40-Avi-6H-BirA |
result | 1393 | Nhe1+kpn1 | 564bp | Amp | PCR | sequencing insert is good, subclone to 3232 | #5 | sub | |||
| 3191 | pT7A3-GST-Avitag |
1851 | K | Annealing | sequencing good, completed |
GS Made | |||||||
| 3232 | pCMV5-Avi-6H-BirA |