Cloning of alpaca VHH cDNA

From ELN
Revision as of 16:26, 8 July 2018 by Paul.xu (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

RT primers: Camelus, Lama

ATGGAGAGGACGTCCTTGGGT

TTCGGGGGGAAGAYRAAGAC


PCR

AlpVh-L (GGTGGTCCTGGCTGC); 
AlpVh-F1 (GATCGCCGGCCAGKTGCAGCTCGTGGAGTCNGGNGG); 
AlpVHH-R1 (GATCACTAGTGGGGTCTTCGCTGTGGTGCG) :  primes within the short hinge coding region at the same site as Lam07; 
and AlpVHH-R2 (GATCACTAGTTTGTGGTTTTGGTGTCTTGGG)

use E. coli Rosetta-gami for VHH antibody expression

Retrieved from "https://eln.excellgen.com/index.php?title=Cloning_of_alpaca_VHH_cDNA&oldid=18606"