Cloning of alpaca VHH cDNA

RT primers: Camelus, Lama

ATGGAGAGGACGTCCTTGGGT

TTCGGGGGGAAGAYRAAGAC

PCR

AlpVh-L (GGTGGTCCTGGCTGC); 
AlpVh-F1 (GATCGCCGGCCAGKTGCAGCTCGTGGAGTCNGGNGG); 
AlpVHH-R1 (GATCACTAGTGGGGTCTTCGCTGTGGTGCG) :  primes within the short hinge coding region at the same site as Lam07; 
and AlpVHH-R2 (GATCACTAGTTTGTGGTTTTGGTGTCTTGGG)

use E. coli Rosetta-gami for VHH antibody expression