3244 pERD-sfGFP
Cloning of 3244 pERD-sfGFP
[edit]Purpose: Cloning of 3244 pERD-sfGFP
Date: 07, 25, 2018
Notebook:
Page:
Clone ID: 3244
Experimenters: yijia
Results
[edit]Cloning
[edit]PCR from 128 pET30b-sfGFP
PCR of 3244
Size:780bp
method:
first: 1 ul of 1 uM F3--R2, 10 cycles;
2nd: 1 ul of 1 uM F2--R2, 10 cycles;
3nd 1ul of 10 uM 3244-F1--3244-R1 25 cycles;
clone into 3242 pERD using Nde1-HindIII;
3244 PCR
Vector digestion
Vector is 3242
buffer:Fastdigest
ENzymes:Nde1+HindIII
Time:1hour
Colony screen
[edit]3244 PCR screening
Miniprep digestion998_129_3244
Miniprep digestion 2_3244_129_998
