3244 pERD-sfGFP

Cloning of 3244 pERD-sfGFP

Purpose: Cloning of 3244 pERD-sfGFP

Date: 07, 25, 2018

Notebook: 

Page:

Clone ID: 3244

Experimenters: yijia

Results

Cloning

PCR from 128 pET30b-sfGFP

PCR of 3244

Size:780bp

method:

first: 1 ul of 1 uM F3--R2, 10 cycles;

2nd: 1 ul of 1 uM F2--R2, 10 cycles;

3nd 1ul of 10 uM 3244-F1--3244-R1 25 cycles;

clone into 3242 pERD using Nde1-HindIII;

3244 PCR

Vector digestion

Vector is 3242

buffer:Fastdigest

ENzymes:Nde1+HindIII

Time:1hour

 Colony screen

3244 PCR screening

Miniprep digestion998_129_3244

Miniprep digestion 2_3244_129_998