Ni Buffer A

20 L Buffer A for Ni resin

50 mM Tris-HCl, pH 8.0, 500 mM NaCl,  5 mM imidazole, 1 mM b-ME, 0.001 mM EDTA

2.5 L 4 M NaCl, or 584 g solid NaCl

127 g Trizma HCL

24g Trizma Base

40 ul 0.5 M EDTA

1.4 ml beta-ME

6 g imidazole

Add 4 L water to dissolve.

Add 16 L ddH20 to make 20 L

20 L Lysis Buffer for Ni resin

50 mM Tris-HCl, pH8.0, 500 mM NaCl,  5 mM imidazole, 1 mM b-ME, 0.001 mM EDTA, 0.1% Triton X-100

2.5 L 4 M NaCl, or 584 g solid NaCl

127 g Trizma HCL

24g Trizma Base

40 ul 0.5 M EDTA

1.4 ml beta-ME

6 g imidazole
20 g Triton X-100

Add 4 L water to dissolve.

Add 16 L ddH20 to make 20 L

Other Buffers

1, 4 M NaCl, 4 L

Dissolve 234 g NaCl in 4 L ddH2O

2, Tris-HCl, 1 M, 1 L

Dissolve 127 g Trizma HCL + 24g Trizma Base in 1 L ddH2O

3, 0.5 M EDTA, 1 L

dissolve 115 g in 1 L ddH2O