Ni Buffer A
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20 L Buffer A for Ni resin
[edit]50 mM Tris-HCl, pH 8.0, 500 mM NaCl, 5 mM imidazole, 1 mM b-ME, 0.001 mM EDTA
2.5 L 4 M NaCl, or 584 g solid NaCl
127 g Trizma HCL
24g Trizma Base
40 ul 0.5 M EDTA
1.4 ml beta-ME
6 g imidazole
Add 4 L water to dissolve.
Add 16 L ddH20 to make 20 L
20 L Lysis Buffer for Ni resin
[edit]50 mM Tris-HCl, pH8.0, 500 mM NaCl, 5 mM imidazole, 1 mM b-ME, 0.001 mM EDTA, 0.1% Triton X-100
2.5 L 4 M NaCl, or 584 g solid NaCl
127 g Trizma HCL
24g Trizma Base
40 ul 0.5 M EDTA
1.4 ml beta-ME
6 g imidazole
20 g Triton X-100
Add 4 L water to dissolve.
Add 16 L ddH20 to make 20 L
Other Buffers
[edit]1, 4 M NaCl, 4 L
Dissolve 234 g NaCl in 4 L ddH2O
2, Tris-HCl, 1 M, 1 L
Dissolve 127 g Trizma HCL + 24g Trizma Base in 1 L ddH2O
3, 0.5 M EDTA, 1 L
dissolve 115 g in 1 L ddH2O