135 pQE-80L-HTNCre HTNCre

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Subcloning:

For 134: vector 212 and  insert 1707: Nco1 + Xho1, wrong, should use Nde1 + Not1

For 136: Vector 2038 and insert  129, Nco1 - Xho1 


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Miniprep digestion

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135 miniprep digestion

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