135 pQE-80L-HTNCre HTNCre

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Subcloning:

For 134: vector 212 and insert 1707: Nco1 + Xho1, wrong, should use Nde1 + Not1

For 136: Vector 2038 and insert 129, Nco1 - Xho1

Miniprep digestion

135 miniprep digestion

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Cloning