Packagingmix optimization 2

From ELN
Revision as of 15:23, 5 February 2011 by Paul.xu (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Notebook #

Page #: 001430



1 Day after transfection



2 days after transfection



Vector
 Packaging mix
 Transfection Reagent
1
 -
 -
 -
2
 -
 Sigma
 PolyExpress
3
 TurboGFP
 Sigma
 PolyExpress
4
 TurboGFP
 1:1
 PolyExpress
5
 TurboGFP
 1:3
 PolyExpress
6
 TurboGFP
 1:5
 PolyExpress
7
 TurboGFP
 1:10
 PolyExpress
8
 TurboGFP
 Sigma
 FuGene
9
 TurboGFP
 1:3
 FuGene
10
 pSMPUW-EGFP
 1:3 PolyExpress
11
 pSMPUW-EGFP
 Sigma
 PolyExpress
12
 pSMPUW-EGFP
 Sigma
 FuGene


Fugene: 1.2 ul

PolyExpress: 1.2 ul

Sigma TurboGFP:0.2 ug (1ul of 200 ng/ul)

pSMPUW-EGFP: 0.2 ug (1ul of 200 ng/ul)

Packaging mix: 2 ul (Sigma or homemade, pVSVG/pGag-Pol)


--Paul.xu 16:49, 28 January 2011 (EST)

1 day post infection


--Paul.xu 11:01, 30 January 2011 (EST)



2 days after infection


3 days after infection
--Paul.xu 10:38, 3 February 2011 (EST)


4 days after infection

5 days after infection
--Paul.xu 14:23, 5 February 2011 (EST)

Retrieved from "https://eln.excellgen.com/index.php?title=Packagingmix_optimization_2&oldid=199"