Packagingmix optimization 2
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Notebook #
Page #: 001430
1 Day after transfection
2 days after transfection
| Vector |
Packaging mix |
Transfection Reagent | |
| 1 |
- |
- |
- |
| 2 |
- |
Sigma |
PolyExpress |
| 3 |
TurboGFP |
Sigma |
PolyExpress |
| 4 |
TurboGFP |
1:1 |
PolyExpress |
| 5 |
TurboGFP |
1:3 |
PolyExpress |
| 6 |
TurboGFP |
1:5 |
PolyExpress |
| 7 |
TurboGFP |
1:10 |
PolyExpress |
| 8 |
TurboGFP |
Sigma |
FuGene |
| 9 |
TurboGFP |
1:3 |
FuGene |
| 10 |
pSMPUW-EGFP |
1:3 | PolyExpress |
| 11 |
pSMPUW-EGFP |
Sigma |
PolyExpress |
| 12 |
pSMPUW-EGFP |
Sigma |
FuGene |
Fugene: 1.2 ul
PolyExpress: 1.2 ul
Sigma TurboGFP:0.2 ug (1ul of 200 ng/ul)
pSMPUW-EGFP: 0.2 ug (1ul of 200 ng/ul)
Packaging mix: 2 ul (Sigma or homemade, pVSVG/pGag-Pol)
--Paul.xu 16:49, 28 January 2011 (EST)
1 day post infection
--Paul.xu 11:01, 30 January 2011 (EST)
2 days after infection
3 days after infection
--Paul.xu 10:38, 3 February 2011 (EST)
4 days after infection
5 days after infection
--Paul.xu 14:23, 5 February 2011 (EST)