SpyCatcher Spytag

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Summary of Catcher-Tag technologies and their development.

Catcher Tag Tag Sequence Description Publication Year
SpyCatcher SpyTag AHIVMVDAYKPTK Original Catcher-Tag technology. 2012
SpyCatcher
ΔN1ΔC1
SpyTag AHIVMVDAYKPTK Minimal SpyCatcher construct that still binds efficiently to SpyTag. 2014
SpyLigase 2 SpyTag AHIVMVDAYKPTK Rationally engineered system for ligating two peptides. 2014
KTag ATHIKFSKRD
SnoopCatcher SnoopTag KLGDIEFIKVNK Orthogonal technology to SpyCatcher. 2016
SpyCatcher002 SpyTag002 VPTIVMVDAYKRYK Improved SpyCatcher-SpyTag system with faster reaction rate. 2017
SnoopLigase 2 SnoopTagJr KLGSIEFIKVNK Rationally engineered system for ligating two peptides. 2018
DogTag DIPATYEFTDGKHYITNEPIPPK
SpyDock SpyTag002 VPTIVMVDAYKRYK Protein affinity purification system (Spy&Go) based on SpyCatcher.