1074 pET28b-IHF

Cloning of 1074 pET28b-IHF

Purpose: Cloning of 1074 pET28b-IHF

Date: 2012-4-13---2012-4-16

Notebook:0

Page:

Clone ID:1074

Experimenters: yingying

Results

subcloning

	band 1	band 2
	405 pMCSG13-IHF	pET28b
enzymes	Nco1 + Xho1

Colony Screening

Date: 2012-4-16

Make miniprep and digest with Nco1 + Xho1.

Result intepretation/Summary

• <br>

Conclusion

<br>

<br>

<br>

<br>

Protein Expression

induction, 37 oC 5 hours

Lane	ID	Name	Pi, MW	Induction	Sample	Expression
1	1708	pET28b-scIHF2			S
2	1203	pET42b-scIHF2			S
3	1074	pET28b-IHFab			S
4	1186	pDB.His.MBP-Xis			S
5	406	pET28b-Int			S
6	1185	pDB.His.MBP-Int
7	1708	pET28b-scIHF2	9.60 / 22.8		P	Y
8	1203	pET42b-scIHF2	8.38 / 54.2		P	Y
9	1074	pET28b-IHFab	9.20 / 25.3		P	N
10	1186	pDB.His.MBP-Xis	6.62 / 52.1		P	Y
11	406	pET28b-Int	9.53 / 42.5		P	N
12	1185	pDB.His.MBP-Int	7.33 / 83.7		P	Y
M		PageRuler Plus	250, 130, 00, 70, 55, 35, 25, 15, 10