1074 pET28b-IHF
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Cloning of 1074 pET28b-IHF
[edit]Purpose: Cloning of 1074 pET28b-IHF
Date: 2012-4-13---2012-4-16
Notebook:0
Page:
Clone ID:1074
Experimenters: yingying
Results
[edit]subcloning
[edit]
| band 1 |
band 2 | |
| 405 pMCSG13-IHF |
pET28b | |
| enzymes |
Nco1 + Xho1 | |
Colony Screening
[edit]Date: 2012-4-16
Make miniprep and digest with Nco1 + Xho1.
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
<br>
<br>
<br>
Protein Expression
[edit]induction, 37 oC 5 hours
| Lane | ID | Name | Pi, MW | Induction | Sample | Expression |
| 1 | 1708 | pET28b-scIHF2 |
S | |||
| 2 | 1203 | pET42b-scIHF2 |
S | |||
| 3 | 1074 | pET28b-IHFab |
S | |||
| 4 | 1186 | pDB.His.MBP-Xis |
S | |||
| 5 | 406 |
pET28b-Int |
S | |||
| 6 | 1185 | pDB.His.MBP-Int |
||||
| 7 | 1708 |
pET28b-scIHF2 |
9.60 / 22.8 |
P | Y | |
| 8 | 1203 |
pET42b-scIHF2 |
8.38 / 54.2 |
P | Y | |
| 9 | 1074 |
pET28b-IHFab |
9.20 / 25.3 |
P |
N | |
| 10 | 1186 |
pDB.His.MBP-Xis |
6.62 / 52.1 |
P |
Y | |
| 11 | 406 |
pET28b-Int |
9.53 / 42.5 |
P |
N | |
| 12 | 1185 |
pDB.His.MBP-Int |
7.33 / 83.7 |
P |
Y | |
| M | PageRuler Plus | 250, 130, 00, 70, 55, 35, 25, 15, 10 | ||||
