E coli genome engineering

From ELN
Revision as of 09:47, 24 October 2019 by Paul.xu (talk | contribs) (Created page with "safe harbor: between ybhD ~ybhH TTATTACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTA...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

safe harbor:

between ybhD ~ybhH

TTATTACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTA

https://www.atum.bio/eCommerce/cas9/input select: Ecoli, NGG, Wild-type Cas9, rRNA offset 4 to 10, target sequence, showing 3 , and this one is good:  TGAATTTAATTACTTTTTTA, then copy the top strand : ACTTAAATTAATGAAAAAAT, check it on snapgene viewer

Retrieved from "https://eln.excellgen.com/index.php?title=E_coli_genome_engineering&oldid=20307"