E coli genome engineering

safe harbor:

between ybhD ~ybhH

TTATTACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTA

https://www.atum.bio/eCommerce/cas9/input select: Ecoli, NGG, Wild-type Cas9, rRNA offset 4 to 10, target sequence, showing 3 , and this one is good:  TGAATTTAATTACTTTTTTA, then copy the top strand : ACTTAAATTAATGAAAAAAT, check it on snapgene viewer