2640
11/1/2019
Use Nico21(DE3)pRare2pLysS, 15 ml, induced with 1 mM IPTG, 37 oC 5 hours
cre in Nico21(DE3)pLysN, (from freezer, not freshly transformed) 8L, induce 37 oC 6 hours, 0.15% arabinose., use 50 ml Ni column
| Lane | 1 | MW | Expression | |
| 1 | 1078 | pET28b-scIHF2, soluble |
22.8 | N |
| 2 | 406 | pET28b-Int, soluble |
42.5 | N |
| 3 | 1185 | pDB.His.MBP-Int, soluble |
83.7 | Y |
| 4 | 2640 | pCold-MBP-(Ent-TEV)-scIHF, soluble |
66.9 | N |
| 5 | 2640 | pCold-MBP-(Ent-TEV)-scIHF, inclusion body |
66.9 |
Y |
| 6~12 | 1010 | Cre, |
could be low | |
| 13 | 1078 | pET28b-scIHF2 |
22.8 |
N |
| 14 | 406 | pET28b-Int |
42.5 |
N |
| M | PageRuler Plus |
