2640

11/1/2019

Use Nico21(DE3)pRare2pLysS, 15 ml, induced with 1 mM IPTG, 37 oC 5 hours

cre in Nico21(DE3)pLysN, (from freezer, not freshly transformed) 8L, induce 37 oC 6 hours, 0.15% arabinose., use 50 ml Ni column

Lane	1		MW	Expression
1	1078	pET28b-scIHF2, soluble	22.8	N
2	406	pET28b-Int, soluble	42.5	N
3	1185	pDB.His.MBP-Int, soluble	83.7	Y
4	2640	pCold-MBP-(Ent-TEV)-scIHF, soluble	66.9	N
5	2640	pCold-MBP-(Ent-TEV)-scIHF, inclusion body	66.9	Y
6~12	1010	Cre,		could be low
13	1078	pET28b-scIHF2	22.8	N
14	406	pET28b-Int	42.5	N
M		PageRuler Plus