2779

PCR from genomic DNA using superhifi II enzyme mix

6/6/2020

PCR again from genomic DNA

20200616 NdeI+HindIII, buffer2.1 ,37oC, 1hour 

2779 gibson

20200617 to get the vector from #483 digest with NdeI+HindIII in Buffer 2, 37oC , 40min

Protein Expression

RTENOTITLE

Lane	Name	Desc
1	N protein 2 ul
2	N protein 5 ul
3	S protein 5 ul	BEI
4	RBD  5 ul	BEI
5	2779 Turner 37 oC 2 hours, 7.5 ml, 0.2% rhamnose
6	2779 NEB 37 oC 2 hours,  7.5 ml, 0.2% rhamnose
7	2746 1mM IPTG
8	2723
9	2779 NEB 25 oC o/n  7.5 ml, 0.2% rhamnose	cell dead
10	2779 NEB 25 oC o/n  7.5 ml, 0.2% rhamnose	cell dead