2779
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PCR from genomic DNA using superhifi II enzyme mix
6/6/2020 
PCR again from genomic DNA
20200616 NdeI+HindIII, buffer2.1 ,37oC, 1hour
2779 gibson
[edit]20200617 to get the vector from #483 digest with NdeI+HindIII in Buffer 2, 37oC , 40min
Protein Expression
[edit]
| Lane | Name | Desc |
| 1 | N protein 2 ul | |
| 2 | N protein 5 ul | |
| 3 | S protein 5 ul | BEI |
| 4 | RBD 5 ul | BEI |
| 5 | 2779 Turner 37 oC 2 hours, 7.5 ml, 0.2% rhamnose | |
| 6 | 2779 NEB 37 oC 2 hours, 7.5 ml, 0.2% rhamnose | |
| 7 | 2746 1mM IPTG | |
| 8 | 2723 | |
| 9 | 2779 NEB 25 oC o/n 7.5 ml, 0.2% rhamnose | cell dead |
| 10 | 2779 NEB 25 oC o/n 7.5 ml, 0.2% rhamnose | cell dead |
