2889
T7-VEE-FLuc
2889-F: TCATATGTAAGGCGCGCCACCATGGAAGACGCCAAAAACA 2889-R: ttGCGGCCGCGGCCGGCTTACAATTTGGACTTTCCG (CGGAAAGTCCAAATTGTAAGCCGGCCGCGGCCGCaa) clone into pMiniT, then subclone using Nde1-Not1 >201228-13_O07_1F85DAA023.ab1 1192, 12/29/2020 seq with pminiT-F NNNNNNNTNNNNNNNCGACCGATTGTTAGGTAATCGTCACCTGCAGGAAG GTTTAAACGCATTTAGGTGACACTATAGAAGTGTGTATCGCTCGAGGGAT CCGAATTCAGGAGGTAAAAACCATGATGCGGCCGCGGCCGGCTTACAATT TGGACTTTCCGCCCTTCTTGGCCTTTATGAGGATCTCTCTGATTTTTCTT GCGTCGAGTTTTCCGGTAAGACCTTTCGGTACTTCGTCCACAAACACAAC TCCTCCGCGCAACTTTTTCGCGGTTGTTACTTGACTGGCGACGTAATCCA CGATCTCTTTTTCCGTCATCGTCTTTCCGTGCTCCAAAACAACAACGGCG GCGGGAAGTTCACCGGCGTCATCGTCGGGAAGACCTGCCACGCCCGCGTC GAAGATGTTGGGGTGTTGTAACAATATCGATTCCAATTCAGCGGGGGCCA CCTGATATCCTTTGTATTTAATTAAAGACTTCAAGCGGTCAACTATGAAG AAGTGTTCGTCTTCGTCCCAGTAAGCTATGTCTCCAGAATGTAGCCATCC ATCCTTGTCAATCAAGGCGTTGGTCGCTTCCGGATTGTTTACATAACCGG ACATAATCATAGGTCCTCTGACACATAATTCGCCTCTCTGATTAACGCCC AGCGTTTTCCCGGTATCCAGATCCACAACCTTCGCTTCAAAAAATGGAAC AACTTTACCGACCGCGCCCGGTTTATCATCCCCCTCGGGTGTAATCAGAA TAGCTGATGTAGTCTCAGTGAGCCCATATCCTTGTCGTATCCCTGGAAGA TGGAAGCGTTTTGCAACCGCTTCCCCGACTTCTTTCGAAAGAGGTGCGCC CCCAGAAGCAATTTCGTGTAAATTAGATAAATCGTATTTGTCAATCAGAG TGCTTTTGGCGAAGAATGAAAATAGGGTTGGTACTAGCAACGCACTTTGA ATTTTGTAATCCTGAAGGGATCGTAAAAACAGCTCTTCTTCAAATCTATA CATTAAGACGACTCGAAATCCACATATCAAATATCCGAGTGTAGTAAACA TTCCAAAACCGTGATGGAATGGAACAACACTTAAAATCGCAGTATCCGGA ATGATTTGATTGCCAAAAATAGGATCTCTGGCATGCGAGAATCTGACGCA GGCAGTTCTATGCGGAAGGGCCNACCCTTAGGTAACCCAGTA rv TACTGGGTTACCTAAGGGTNGGCCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATGTGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAGGATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAAAGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAACCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGATACCGGGAAAACGCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGATTATGTCCGGTTATGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCTGGAGACATAGCTTACTGGGACGAAGACGAACACTTCTTCATAGTTGACCGCTTGAAGTCTTTAATTAAATACAAAGGATATCAGGTGGCCCCCGCTGAATTGGAATCGATATTGTTACAACACCCCAACATCTTCGACGCGGGCGTGGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCCCGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAAAAAGAGATCGTGGATTACGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCGGAGGAGTTGTGTTTGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAAGGCCAAGAAGGGCGGAAAGTCCAAATTGTAA GCCGGCC Not1 GCGGCCGCA TCATGGTTTTTACCTCCTGAATTCGGATCCCTCGAGCGATACACACTTCTATAGTGTCACCTAAATGCGTTTAAACCTTCCTGCAGGTGACGATTACCTAACAATCGGTCGNNNNNNNANNNNNNN Score Expect Identities Gaps Strand 1960 bits(1061) 0.0 1065/1067(99%) 1/1067(0%) Plus/Plus Query 1 TACTGGGTTACCTAAGGGT-NGGCCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCA 59 ||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||| Sbjct 629 TACTGGGTTACCTAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCA 688 Query 60 TGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGT 119 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 689 TGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGT 748 Query 120 TCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATGTGGATTTCG 179 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 749 TCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATGTGGATTTCG 808 Query 180 AGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAGGATTACAA 239 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 809 AGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAGGATTACAA 868 Query 240 AATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGAT 299 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 869 AATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGAT 928 Query 300 TGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAA 359 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 929 TGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAA 988 Query 360 AGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGG 419 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 989 AGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGG 1048 Query 420 GCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAACCGGGCGC 479 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1049 GCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAACCGGGCGC 1108 Query 480 GGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGATACCGGGAAAAC 539 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1109 GGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGATACCGGGAAAAC 1168 Query 540 GCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGATTATGTCCGGTTA 599 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1169 GCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGATTATGTCCGGTTA 1228 Query 600 TGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCTGG 659 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1229 TGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCTGG 1288 Query 660 AGACATAGCTTACTGGGACGAAGACGAACACTTCTTCATAGTTGACCGCTTGAAGTCTTT 719 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1289 AGACATAGCTTACTGGGACGAAGACGAACACTTCTTCATAGTTGACCGCTTGAAGTCTTT 1348 Query 720 AATTAAATACAAAGGATATCAGGTGGCCCCCGCTGAATTGGAATCGATATTGTTACAACA 779 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1349 AATTAAATACAAAGGATATCAGGTGGCCCCCGCTGAATTGGAATCGATATTGTTACAACA 1408 Query 780 CCCCAACATCTTCGACGCGGGCGTGGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCC 839 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1409 CCCCAACATCTTCGACGCGGGCGTGGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCC 1468 Query 840 CGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAAAAAGAGATCGTGGATTA 899 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1469 CGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAAAAAGAGATCGTGGATTA 1528 Query 900 CGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCGGAGGAGTTGTGTTTGTGGACGA 959 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1529 CGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCGGAGGAGTTGTGTTTGTGGACGA 1588 Query 960 AGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAA 1019 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1589 AGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAA 1648 Query 1020 GGCCAAGAAGGGCGGAAAGTCCAAATTGTAAGCCGGCCGCGGCCGCA 1066 ||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1649 GGCCAAGAAGGGCGGAAAGTCCAAATTGTAAGCCGGCCGCGGCCGCA 1695 >210108-18_A05_1F85DAA019.ab1 1225, 1/9/2021 2889 in MiniT2 seq with pminiT-R primer no good missing TCATATGTA Nde1 site NNNNNNTNNNNNGTATTTAGAAATAAACAAATAGGGGTTCCGCCCGCGAA ATTAATACGACTCACTATAGGGAGACGCACAT Not1 GCGGCCGC CTCGAGAATT CTGACGTCTTAATTAATTATTATC AGGCGCGCCACCATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGAGGATGGAACCG CTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGA ACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGA ATACTTCGAAATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGC TGAATACAAATCACAGAATCGTCGTATGCAGTGAAAACTCTCTTCAATTC TTTATGCCGGTGTTGGGCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGC GAACGACATTTATAATGAACGTGAATTGCTCAACAGTATGAACATTTCGC AGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAAC GTGCAAAAAAAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAA AACGGATTACCAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATC TACCTCCCGGTTTTAATGAATACGATTTTGTACCAGAGTCCTTTGATCGT GACAAAACAATTGCACTGATAATGAATTCCTCTGGATCTACTGGGTTACC TAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCATG CCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTA AGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATA TTTGATATGTGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGC TGTTTTTACGATCCCTTCAGGATTACAAAATTCAAAGTGCGTTGCTAGTA CCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGATTGACAAATACGA TTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAAAG AAGTCGGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACA AGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCCAAG GGGGAAGATAAAACCGGGCGCGGGC Alignment statistics for match #1 Score Expect Identities Gaps Strand 1989 bits(1077) 0.0 1089/1094(99%) 3/1094(0%) Plus/Plus Query 130 AGGCGCGCCACCATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGA 189 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 20 AGGCGCGCCACCATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGA 79 Query 190 GGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGG 249 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 80 GGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGG 139 Query 250 AACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGAATACTTCGA 309 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 140 AACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGAATACTTCGA 199 Query 310 AATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAAT 369 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 200 AATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAAT 259 Query 370 CGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCGTTATTTAT 429 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 260 CGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCGTTATTTAT 319 Query 430 CGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAGTAT 489 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 320 CGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAGTAT 379 Query 490 GAACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAA 549 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 380 GAACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAA 439 Query 550 CGTGCaaaaaaaaTTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTA 609 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 440 CGTGCAAAAAAAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTA 499 Query 610 CCAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGA 669 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 500 CCAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGA 559 Query 670 ATACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACAATTGCACTGATAATGAATTC 729 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 560 ATACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACAATTGCACTGATAATGAATTC 619 Query 730 CTCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAG 789 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 620 CTCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAG 679 Query 790 ATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTT 849 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 680 ATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTT 739 Query 850 AAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATG 909 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 740 AAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATG 799 Query 910 TGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCA 969 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 800 TGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCA 859 Query 970 GGATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAG 1029 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 860 GGATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAG 919 Query 1030 CACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACC 1089 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 920 CACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACC 979 Query 1090 TCTTTCGAAAGAAGTCGGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGAC 1149 |||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||| Sbjct 980 TCTTTCGAAAGAAGTC-GGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGAC 1038 Query 1150 AAGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCCAAGGGGGAAGAT 1209 ||||||||||||||||||||||||||||||||||||||||||||||| ||||||| ||| Sbjct 1039 AAGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCG-AGGGGGATGAT 1097 Query 1210 AAAACCGGGCGCGG 1223 ||| |||||||||| Sbjct 1098 AAA-CCGGGCGCGG 1110 PCR again using 10 uM 2895-F3 TCTAGTCCGCCAAGTCTAGcatatgtaaGGCGCGCCCAC 1uL 2889-F: TCATATGTAAGGCGCGCCACCATGGAAGACGCCAAAAACA and 10 uM 2889-R: ttGCGGCCGCGGCCGGCTTACAATTTGGACTTTCCG (CGGAAAGTCCAAATTGTAAGCCGGCCGCGGCCGCaa) clone into pMiniT, then subclone using Nde1-Not1 >210119-09_E07_1F85DAA000.ab1 1347, 1/20/2021 2889 in pVEE good NNNNNNNNNNNTNNGCTACCTGATGGACTACGACATAGTCTAGTCCGCCA AGTCTAG Nde1 CATATG TAAGGCGC Kozak GCCACCATGGAAGACGCCAAAAACATAAA GAAAGGCCCGGCGCCATTCTATCCTCTAGAGGATGGAACCGCTGGAGAGC AACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGAACAATTGCT TTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGAATACTTCGA AATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAA ATCACAGAATCGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCG GTGTTGGGCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGCGAACGACAT TTATAATGAACGTGAATTGCTCAACAGTATGAACATTTCGCAGCCTACCG TAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAACGTGCAAAAA AAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTA CCAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCG GTTTTAATGAATACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACA ATTGCACTGATAATGAATTCCTCTGGATCTACTGGGTTACCTAAGGGTGT GGCCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCATGCCAGAGATC CTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGTT CCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATG TGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTAC GATCCCTTCAGGATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTA TTTTCATTCTTCGCCAAAAGCACTCTGATTGACAAATACGATTTATCTAA TTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAAAGAAGTCGGGG AAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGGG CTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAA ACCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTGGGGA TCTGGATACCGGGAAAAANCTGGGCGTTAATCAGAAGGGGAATTAGGNTC AAAAGACCTTGAATTAGTCCGGTTTGTAAACANTCCGGAAGNNCCACCCC TTGATGANAGGNNGGNGGGTNNNTNNGGGAAANNNNTTTANNGGAAA Score Expect Identities Gaps Strand 2178 bits(1179) 0.0 1221/1242(98%) 9/1242(0%) Plus/Plus CATATGTAAGGCGCGCCACCATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGAG 131 |||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| CATATG-AAGGCGCGCCACCATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGAG 98 Query 132 GATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGA 191 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 99 GATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGA 158 Query 192 ACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGAATACTTCGAA 251 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 159 ACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGAATACTTCGAA 218 Query 252 ATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAATC 311 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 219 ATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAATC 278 Query 312 GTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCGTTATTTATC 371 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 279 GTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCGTTATTTATC 338 Query 372 GGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAGTATG 431 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 339 GGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAGTATG 398 Query 432 AACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAAC 491 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 399 AACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAAC 458 Query 492 GTGCaaaaaaaaTTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTAC 551 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 459 GTGCAAAAAAAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTAC 518 Query 552 CAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGAA 611 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 519 CAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGAA 578 Query 612 TACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACAATTGCACTGATAATGAATTCC 671 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 579 TACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACAATTGCACTGATAATGAATTCC 638 Query 672 TCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAGA 731 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 639 TCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAGA 698 Query 732 TTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTA 791 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 699 TTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTA 758 Query 792 AGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATGT 851 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 759 AGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATGT 818 Query 852 GGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAG 911 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 819 GGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAG 878 Query 912 GATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGC 971 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 879 GATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGC 938 Query 972 ACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCT 1031 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 939 ACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCT 998 Query 1032 CTTTCGAAAGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAA 1091 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 999 CTTTCGAAAGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAA 1058 Query 1092 GGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAA 1151 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1059 GGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAA 1118 Query 1152 CCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTGG-GGATCTGGATACC 1210 |||||||||||||||||||||||||||||||||||||||||||| | ||||||||||||| Sbjct 1119 CCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGATACC 1178 Query 1211 GGGAAAAANCTGGGCGTTAATCAGA-AGGGGAATTA-GGNTCAAAAGACCT-TGAATTA- 1266 ||||||| |||||||||||||||| ||| |||||| | ||| | ||||| ||| ||| Sbjct 1179 GGGAAAACGCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGA-TTAT 1237 Query 1267 GTCCGGTT-TGTAAACANTCCGGAAGNN-CCACC-CCTTGAT 1305 |||||||| |||||||| |||||||| ||| | ||||||| Sbjct 1238 GTCCGGTTATGTAAACAATCCGGAAGCGACCAACGCCTTGAT 1279 Range 2: 1 to 38GraphicsNext MatchPrevious MatchFirst Match Alignment statistics for match #2 Score Expect Identities Gaps Strand 65.8 bits(35) 4e-14 38/39(97%) 1/39(2%) Plus/Plus Query 58 96 |||||| |||||||||||||||||||||||||||||||| Sbjct 1 38