2889

T7-VEE-FLuc

2889-F:
TCATATGTAAGGCGCGCCACCATGGAAGACGCCAAAAACA
2889-R:
ttGCGGCCGCGGCCGGCTTACAATTTGGACTTTCCG
(CGGAAAGTCCAAATTGTAAGCCGGCCGCGGCCGCaa)
clone into pMiniT, then subclone using Nde1-Not1



>201228-13_O07_1F85DAA023.ab1 1192, 12/29/2020
seq with pminiT-F
NNNNNNNTNNNNNNNCGACCGATTGTTAGGTAATCGTCACCTGCAGGAAG
GTTTAAACGCATTTAGGTGACACTATAGAAGTGTGTATCGCTCGAGGGAT
CCGAATTCAGGAGGTAAAAACCATGATGCGGCCGCGGCCGGCTTACAATT
TGGACTTTCCGCCCTTCTTGGCCTTTATGAGGATCTCTCTGATTTTTCTT
GCGTCGAGTTTTCCGGTAAGACCTTTCGGTACTTCGTCCACAAACACAAC
TCCTCCGCGCAACTTTTTCGCGGTTGTTACTTGACTGGCGACGTAATCCA
CGATCTCTTTTTCCGTCATCGTCTTTCCGTGCTCCAAAACAACAACGGCG
GCGGGAAGTTCACCGGCGTCATCGTCGGGAAGACCTGCCACGCCCGCGTC
GAAGATGTTGGGGTGTTGTAACAATATCGATTCCAATTCAGCGGGGGCCA
CCTGATATCCTTTGTATTTAATTAAAGACTTCAAGCGGTCAACTATGAAG
AAGTGTTCGTCTTCGTCCCAGTAAGCTATGTCTCCAGAATGTAGCCATCC
ATCCTTGTCAATCAAGGCGTTGGTCGCTTCCGGATTGTTTACATAACCGG
ACATAATCATAGGTCCTCTGACACATAATTCGCCTCTCTGATTAACGCCC
AGCGTTTTCCCGGTATCCAGATCCACAACCTTCGCTTCAAAAAATGGAAC
AACTTTACCGACCGCGCCCGGTTTATCATCCCCCTCGGGTGTAATCAGAA
TAGCTGATGTAGTCTCAGTGAGCCCATATCCTTGTCGTATCCCTGGAAGA
TGGAAGCGTTTTGCAACCGCTTCCCCGACTTCTTTCGAAAGAGGTGCGCC
CCCAGAAGCAATTTCGTGTAAATTAGATAAATCGTATTTGTCAATCAGAG
TGCTTTTGGCGAAGAATGAAAATAGGGTTGGTACTAGCAACGCACTTTGA
ATTTTGTAATCCTGAAGGGATCGTAAAAACAGCTCTTCTTCAAATCTATA
CATTAAGACGACTCGAAATCCACATATCAAATATCCGAGTGTAGTAAACA
TTCCAAAACCGTGATGGAATGGAACAACACTTAAAATCGCAGTATCCGGA
ATGATTTGATTGCCAAAAATAGGATCTCTGGCATGCGAGAATCTGACGCA
GGCAGTTCTATGCGGAAGGGCCNACCCTTAGGTAACCCAGTA

rv
TACTGGGTTACCTAAGGGTNGGCCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATGTGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAGGATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAAAGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAACCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGATACCGGGAAAACGCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGATTATGTCCGGTTATGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCTGGAGACATAGCTTACTGGGACGAAGACGAACACTTCTTCATAGTTGACCGCTTGAAGTCTTTAATTAAATACAAAGGATATCAGGTGGCCCCCGCTGAATTGGAATCGATATTGTTACAACACCCCAACATCTTCGACGCGGGCGTGGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCCCGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAAAAAGAGATCGTGGATTACGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCGGAGGAGTTGTGTTTGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAAGGCCAAGAAGGGCGGAAAGTCCAAATTGTAA
GCCGGCC
Not1
GCGGCCGCA
TCATGGTTTTTACCTCCTGAATTCGGATCCCTCGAGCGATACACACTTCTATAGTGTCACCTAAATGCGTTTAAACCTTCCTGCAGGTGACGATTACCTAACAATCGGTCGNNNNNNNANNNNNNN
Score Expect Identities Gaps Strand
1960 bits(1061) 0.0 1065/1067(99%) 1/1067(0%) Plus/Plus
Query 1 TACTGGGTTACCTAAGGGT-NGGCCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCA 59
||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 629 TACTGGGTTACCTAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCA 688

Query 60 TGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGT 119
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 689 TGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGT 748

Query 120 TCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATGTGGATTTCG 179
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 749 TCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATGTGGATTTCG 808

Query 180 AGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAGGATTACAA 239
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 809 AGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAGGATTACAA 868

Query 240 AATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGAT 299
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 869 AATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGAT 928

Query 300 TGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAA 359
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 929 TGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAA 988

Query 360 AGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGG 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 989 AGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGG 1048

Query 420 GCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAACCGGGCGC 479
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1049 GCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAACCGGGCGC 1108

Query 480 GGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGATACCGGGAAAAC 539
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1109 GGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGATACCGGGAAAAC 1168

Query 540 GCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGATTATGTCCGGTTA 599
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1169 GCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGATTATGTCCGGTTA 1228

Query 600 TGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCTGG 659
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1229 TGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCTGG 1288

Query 660 AGACATAGCTTACTGGGACGAAGACGAACACTTCTTCATAGTTGACCGCTTGAAGTCTTT 719
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1289 AGACATAGCTTACTGGGACGAAGACGAACACTTCTTCATAGTTGACCGCTTGAAGTCTTT 1348

Query 720 AATTAAATACAAAGGATATCAGGTGGCCCCCGCTGAATTGGAATCGATATTGTTACAACA 779
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1349 AATTAAATACAAAGGATATCAGGTGGCCCCCGCTGAATTGGAATCGATATTGTTACAACA 1408

Query 780 CCCCAACATCTTCGACGCGGGCGTGGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCC 839
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1409 CCCCAACATCTTCGACGCGGGCGTGGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCC 1468

Query 840 CGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAAAAAGAGATCGTGGATTA 899
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1469 CGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAAAAAGAGATCGTGGATTA 1528

Query 900 CGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCGGAGGAGTTGTGTTTGTGGACGA 959
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1529 CGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCGGAGGAGTTGTGTTTGTGGACGA 1588

Query 960 AGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAA 1019
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1589 AGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAA 1648

Query 1020 GGCCAAGAAGGGCGGAAAGTCCAAATTGTAAGCCGGCCGCGGCCGCA 1066
|||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1649 GGCCAAGAAGGGCGGAAAGTCCAAATTGTAAGCCGGCCGCGGCCGCA 1695


>210108-18_A05_1F85DAA019.ab1 1225, 1/9/2021
2889 in MiniT2 seq with pminiT-R primer

no good missing TCATATGTA Nde1 site


NNNNNNTNNNNNGTATTTAGAAATAAACAAATAGGGGTTCCGCCCGCGAA
ATTAATACGACTCACTATAGGGAGACGCACAT
Not1
GCGGCCGC CTCGAGAATT
CTGACGTCTTAATTAATTATTATC

AGGCGCGCCACCATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGAGGATGGAACCG
CTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGA
ACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGA
ATACTTCGAAATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGC
TGAATACAAATCACAGAATCGTCGTATGCAGTGAAAACTCTCTTCAATTC
TTTATGCCGGTGTTGGGCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGC
GAACGACATTTATAATGAACGTGAATTGCTCAACAGTATGAACATTTCGC
AGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAAC
GTGCAAAAAAAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAA
AACGGATTACCAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATC
TACCTCCCGGTTTTAATGAATACGATTTTGTACCAGAGTCCTTTGATCGT
GACAAAACAATTGCACTGATAATGAATTCCTCTGGATCTACTGGGTTACC
TAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCATG
CCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTA
AGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATA
TTTGATATGTGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGC
TGTTTTTACGATCCCTTCAGGATTACAAAATTCAAAGTGCGTTGCTAGTA
CCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGATTGACAAATACGA
TTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAAAG
AAGTCGGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACA
AGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCCAAG
GGGGAAGATAAAACCGGGCGCGGGC

Alignment statistics for match #1
Score Expect Identities Gaps Strand
1989 bits(1077) 0.0 1089/1094(99%) 3/1094(0%) Plus/Plus
Query 130 AGGCGCGCCACCATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGA 189
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 20 AGGCGCGCCACCATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGA 79

Query 190 GGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGG 249
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 80 GGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGG 139

Query 250 AACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGAATACTTCGA 309
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 140 AACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGAATACTTCGA 199

Query 310 AATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAAT 369
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 200 AATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAAT 259

Query 370 CGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCGTTATTTAT 429
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 260 CGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCGTTATTTAT 319

Query 430 CGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAGTAT 489
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 320 CGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAGTAT 379

Query 490 GAACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAA 549
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 380 GAACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAA 439

Query 550 CGTGCaaaaaaaaTTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTA 609
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 440 CGTGCAAAAAAAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTA 499

Query 610 CCAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGA 669
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 500 CCAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGA 559

Query 670 ATACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACAATTGCACTGATAATGAATTC 729
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 560 ATACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACAATTGCACTGATAATGAATTC 619

Query 730 CTCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAG 789
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 620 CTCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAG 679

Query 790 ATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTT 849
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 680 ATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTT 739

Query 850 AAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATG 909
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 740 AAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATG 799

Query 910 TGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCA 969
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 800 TGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCA 859

Query 970 GGATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAG 1029
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 860 GGATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAG 919

Query 1030 CACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACC 1089
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 920 CACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACC 979

Query 1090 TCTTTCGAAAGAAGTCGGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGAC 1149
|||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
Sbjct 980 TCTTTCGAAAGAAGTC-GGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGAC 1038

Query 1150 AAGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCCAAGGGGGAAGAT 1209
||||||||||||||||||||||||||||||||||||||||||||||| ||||||| |||
Sbjct 1039 AAGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCG-AGGGGGATGAT 1097

Query 1210 AAAACCGGGCGCGG 1223
||| ||||||||||
Sbjct 1098 AAA-CCGGGCGCGG 1110

PCR again using

10 uM 2895-F3 TCTAGTCCGCCAAGTCTAGcatatgtaaGGCGCGCCCAC
1uL 2889-F: TCATATGTAAGGCGCGCCACCATGGAAGACGCCAAAAACA
and
10 uM 2889-R: ttGCGGCCGCGGCCGGCTTACAATTTGGACTTTCCG
(CGGAAAGTCCAAATTGTAAGCCGGCCGCGGCCGCaa)
clone into pMiniT, then subclone using Nde1-Not1
 
>210119-09_E07_1F85DAA000.ab1 1347, 1/20/2021
2889 in pVEE good
NNNNNNNNNNNTNNGCTACCTGATGGACTACGACATAGTCTAGTCCGCCA
AGTCTAG
Nde1
CATATG TAAGGCGC
Kozak
GCCACCATGGAAGACGCCAAAAACATAAA
GAAAGGCCCGGCGCCATTCTATCCTCTAGAGGATGGAACCGCTGGAGAGC
AACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGAACAATTGCT
TTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGAATACTTCGA
AATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAA
ATCACAGAATCGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCG
GTGTTGGGCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGCGAACGACAT
TTATAATGAACGTGAATTGCTCAACAGTATGAACATTTCGCAGCCTACCG
TAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAACGTGCAAAAA
AAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTA
CCAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCG
GTTTTAATGAATACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACA
ATTGCACTGATAATGAATTCCTCTGGATCTACTGGGTTACCTAAGGGTGT
GGCCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCATGCCAGAGATC
CTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGTT
CCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATG
TGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTAC
GATCCCTTCAGGATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTA
TTTTCATTCTTCGCCAAAAGCACTCTGATTGACAAATACGATTTATCTAA
TTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAAAGAAGTCGGGG
AAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGGG
CTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAA
ACCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTGGGGA
TCTGGATACCGGGAAAAANCTGGGCGTTAATCAGAAGGGGAATTAGGNTC
AAAAGACCTTGAATTAGTCCGGTTTGTAAACANTCCGGAAGNNCCACCCC
TTGATGANAGGNNGGNGGGTNNNTNNGGGAAANNNNTTTANNGGAAA


Score Expect Identities Gaps Strand
2178 bits(1179) 0.0 1221/1242(98%) 9/1242(0%) Plus/Plus
CATATGTAAGGCGCGCCACCATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGAG 131
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
CATATG-AAGGCGCGCCACCATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGAG 98

Query 132 GATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGA 191
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 99 GATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGA 158

Query 192 ACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGAATACTTCGAA 251
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 159 ACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGAATACTTCGAA 218

Query 252 ATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAATC 311
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 219 ATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAATC 278

Query 312 GTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCGTTATTTATC 371
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 279 GTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCGTTATTTATC 338

Query 372 GGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAGTATG 431
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 339 GGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAGTATG 398

Query 432 AACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAAC 491
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 399 AACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAAC 458

Query 492 GTGCaaaaaaaaTTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTAC 551
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 459 GTGCAAAAAAAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTAC 518

Query 552 CAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGAA 611
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 519 CAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGAA 578

Query 612 TACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACAATTGCACTGATAATGAATTCC 671
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 579 TACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACAATTGCACTGATAATGAATTCC 638

Query 672 TCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAGA 731
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 639 TCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAGA 698

Query 732 TTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTA 791
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 699 TTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTA 758

Query 792 AGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATGT 851
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 759 AGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATGT 818

Query 852 GGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAG 911
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 819 GGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAG 878

Query 912 GATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGC 971
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 879 GATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGC 938

Query 972 ACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCT 1031
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 939 ACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCT 998

Query 1032 CTTTCGAAAGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAA 1091
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 999 CTTTCGAAAGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAA 1058

Query 1092 GGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAA 1151
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1059 GGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAA 1118

Query 1152 CCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTGG-GGATCTGGATACC 1210
|||||||||||||||||||||||||||||||||||||||||||| | |||||||||||||
Sbjct 1119 CCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGATACC 1178

Query 1211 GGGAAAAANCTGGGCGTTAATCAGA-AGGGGAATTA-GGNTCAAAAGACCT-TGAATTA- 1266
||||||| |||||||||||||||| ||| |||||| | ||| | ||||| ||| |||
Sbjct 1179 GGGAAAACGCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGA-TTAT 1237

Query 1267 GTCCGGTT-TGTAAACANTCCGGAAGNN-CCACC-CCTTGAT 1305
|||||||| |||||||| |||||||| ||| | |||||||
Sbjct 1238 GTCCGGTTATGTAAACAATCCGGAAGCGACCAACGCCTTGAT 1279


Range 2: 1 to 38GraphicsNext MatchPrevious MatchFirst Match
Alignment statistics for match #2
Score Expect Identities Gaps Strand
65.8 bits(35) 4e-14 38/39(97%) 1/39(2%) Plus/Plus
Query 58 96
|||||| ||||||||||||||||||||||||||||||||
Sbjct 1 38